Napp, Jeffrey Unpublished material Microsoft Excel 2003 Spreadsheet These data were collected under NSF Grant # ARC-0722448 ("BEST: Impacts of Sea-ice on the Hydrographic Structure, Nutrients, and Mesozooplankton over the Eastern Bering Sea Shelf") to Dr. G.L. Hunt, Jr., University of Washington. The eastern Bering Sea shelf supports productive marine ecosystems with extraordinarily valuable fisheries and subsistence resources, but sub-arctic seas are predicted to be one of the regions most sensitive to future warming of the world’s oceans. Some of the most direct effects of changing climate will be on the extent, duration and timing of sea-ice over the Bering Sea shelf. Sea-ice controls the timing of the spring phytoplankton bloom, the fate of primary production, water column temperature and salinity, and provides a haul out and molting platform for marine mammals. Thus, the most urgent priority of the Bering Sea Ecosystem Study (BEST) is to examine the role of changing sea-ice conditions on the chemical, physical, and biological characteristics of the ecosystem. The first BEST cruise was scheduled on the USCG Healy in April-May 2007, however, physical observations, water column nutrient chemistry, and zooplankton distribution / abundance were not among the ecosystem components funded in the first call for proposals. Project ARC-0722448 funded by NSF after the first call for BEST proposals filled this gap in chlorophyll collections until the remainder of BEST projects could be assembled in 2008. To document the along- and cross-shelf variability in spring chlorophyll concentrations on the eastern Bering Sea shelf 20070410 20070512 ground condition Complete None planned -180.0 -160.0 65.0000 54.0000 biota phytoplankton chlorophyll phaeopigments oceans phytoplankton chlorophyll phaeopigments None Alaska arctic eastern Bering Sea shelf Bristol Bay St. Lawrence Island Depths upper water column Seasons Spring None None Napp, Jeffrey National Oceanic and Atmospheric Administration mailing and physical

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Seattle WA 98115 USA (206) 526-4148 (206) 526-6723 jeff.napp@noaa.gov not applicable Data are processed using Seabird... Field Samples are from assorted size and types of PVC water bottles on a CTD rosette or carrousel and closed at a known depth. Water is drained from the bottles through a silicon tube into a calibrated PE or PP sample bottle. The bottom is rinsed three times and then filled to the top. After all sample bottles are filled, they are taken to the ship's laboratory where the contents are filtered through a glass fiber filter (nominal pore size 0.7 micron) over low vacuum (<= 7 psi). The filter is folded twice and placed in a 2 ml microcentrifuge tube. The tubes are store in the dark at - 80 deg. C until sample analysis. Lab Pigments are extracted in the dark at - 80 deg C in 6 ml of 90% acetone for 24 hours. They are then removed from the freezer, vortexed, and centrifuged for 5 minutes at 2,000 rpm. The samples are allowed to come to room temperature before measuring their fluorescence before and after the addition of HCl using a calibrated TD-700 fluorometer. Chlorophyll a and phaeopigments are calculated using standard equations. No process steps have been described for this data set Unknown Point Local surface 1.00 meters Attribute values HLY0701_Chlorophyl_final Phytoplankton pigments (chlorophyll and phaeopigment) from water bottle samples Napp, Jeffrey FOCICruise FOCI Program Cruise Number XYYZZ(X=sequential cruise number; YY= ship designation; ZZ= year) See entity type definition source Text Station Sequential Station Number See entity type definition source Integer Haul Sequential Haul Number (N.B. Increment haul number if the operation is performed within 0.5 nm of original station position) See entity type definition source Integer GearAbrv Gear Abbreviation See entity type definition source CTDB Conductivity, Temperature, Depth Instrument with Water Bottles See entity type definition source ShipCruise Ship's Convention for Cruise Name See entity type definition source Text CTDID CTD cast # from which chlorophyll samples were obtained. Concatenated variable: FOCI Cruise + Sequential Station Number + "CTD" + Sequential CTD # See entity type definition source Text GMTTime Greenwich Mean Time See entity type definition source 1899-12-30 00-13-00 1899-12-30 23-26-00 Time Date/Time BottomDepth Depth of Water Column (acoustically determined) See entity type definition source Integer Latitude Latitude (Decimal Degrees) See entity type definition source 54.2438 62.8528 0.0001 Real Longitude Longitude (Decimal Degrees) See entity type definition source -179.4392 -163.9237 0.0001 Real HaulComments Comments Entered By Scientist See entity type definition source Text Performance Scientists Assessment of Sample Quality See entity type definition source good no reservations about quality of sample See entity type definition source quest questionable - some reservations about quality of sample; read Haul Comments to learn why See entity type definition source fail failure during sample collection or processing - do not use these data See entity type definition source GMTDate Greenwich Mean Time Date at Time of Sample Collection See entity type definition source 2007-04-10 00-00-00 2007-05-12 00-00-00 Time Date/Time SampleDepth Depth of Sample Collection See entity type definition source Integer ChloroPerM^3 Chlorophyll a Concentration See entity type definition source 0.0 35.4606 Micrograms per Cubic Meter Real PhaeoPigPerM^3 Phaeophorbide + Phaephytin Concentration See entity type definition source 0.0 11.8799 Micrograms per Cubic Meter Real 20081212 Napp, Jeffrey National Oceanic and Atmospheric Administration mailing and physical

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Seattle WA 98115 USA 206-526-4148 FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999 National Center for Atmospheric Research Earth Observing Laboratory mailing and physical

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Boulder CO 80301 USA 303-497-8154 codiac@ucar.edu stott@ucar.edu