TITLE: Neutral Lipids AOS94 PI: Dr. H. Rodger Harvey Chesapeake Biological Laboratory University of Maryland Center for Environmental Science P.O. Box 38 Solomons, MD 20688 Phone: 410-326-7206 Fax: 410-326-7341 Email: harvey@cbl.umces.edu Website: http://cbl.umces.edu/~harvey/SBI COLLABORATORS: Dr. Robie W. Macdonald Department of Fisheries and Oceans Institute of Ocean Sciences P.O. Box 6000 Sidney, BC, Canada Dr. Mark B. Yunker 7137 Wallace Drive Brentwood Bay, BC, Canada DATA SET OVERVIEW: This data set represents an analysis of neutral lipids in eight surface sediments taken from undisturbed box cores collected during the July-September 1994 Arctic Ocean Section (AOS) cruise aboard the CCGS Louis S. St. Laurent. Sediments were provided sectioned and frozen by a collaborator. Sediments were extracted three times with a mixture of CH2Cl2:MeOH (1:1), and hydrolyzed at 50C for 30 min with a solution of 0.5N KOH in MeOH. After partitioning the fatty acids and neutral lipids with hexande:diethyl ether (9:1), the neutral lipids were derivitized with a solution of bis(trimethylsilyl)trifluoroacetamide and pyridine. Lipids were quantified by capillary gas chromatography and identified by mass spectrometry. For a detailed description of the materials and methods, please see Belicka, L.L., Macdonald, R.W., and Harvey, H.R., 2002. Sources and transport of organic carbon to shelf, slope, and basin surface sediments of the Arctic Ocean. Deep-Sea Research Part I, 49:1463-1483. Information can also be found on our website: http://cbl.umces.edu/~harvey/SBI. This data set consists of an excel workbook with two spreadsheets. The first sheet, labeled STNS, contains information from selected stations from the AOS1994 sample cruise. Information includes station name, latitude, longitude, sediment horizon depth in centimeters, water depth in meters, percentage of organic carbon, percentage of nitrogen, and TOC:TN based on elemental ratios. The second worksheet, labeled NEUTRAL_LIPIDS, contains concentrations (ug lipid/g Org. C) of individual hydrocarbons and alcohols (including sterols) at each of the eight stations. Stations are listed across the top of the sheet, while individual lipids are named in the first column. The page header contains remarks for reading the spreadsheet.