TITLE: HLY-02-03 VPR Data AUTHOR: C. J. Ashjian and S. M. Gallager Department of Biology Woods Hole Oceanographic Institution Woods Hole, MA 02543 Tel: (508)289-3457 Fax: (508)457-2134 Email: cashjian@whoi.edu FUNDING SOURCE AND GRANT NUMBER: NSF OPP-0124766 DATA SET OVERVIEW: Video Plankton Recorder Data Collection and Analysis Data were collected on a cruise (HLY0203) to the Chukchi and Beaufort Seas on the USCGC Healy from July 17 - August 26, 2002 as part of the Shelf Basin Interactions program. The primary sampling instrument was a self- contained, battery powered, single camera Auto Video Plankton Recorder (AutoVPR; SeaScan, Inc.) that was deployed from the stern of the ship in a stainless-steel cage. The AutoVPR records video image frames from a Pulnix TM7 CCD camera at 720 x 640 pixels at 30 Hz as a wavelet compressed data stream together with coincident data from a SeaBird SBE49 CTD operating at 10Hz. The image field of view was calibrated to 1.3 x 1.0 x 6.8 cm (length x width x depth) with a precision of ± 0.02 cm giving an image volume of 8.84 ± 0.34 ml. With a vertical profiling speed of 0.5 ms-1, a new image volume was recorded every 1.6 cm providing nearly contiguous vertical coverage by video information and CTD data at an interval of 5 cm. Profiles were conducted between the surface to 10 m off the bottom or to a maximum depth of 350 m. Immediately following a deployment, video files were down loaded from the internal PC104 computer to a desk top PC via Ethernet. Video files were decompressed and in-focus targets extracted as Regions of Interest (ROI) and written to disk as tagged interchange files (tif) using software provided with the instrument. Extracted images were identified to taxon or particle type manually and merged by time with pressure, temperature, salinity, and density data from the CTD using Matlab (Mathworks, Inc.) routines. Average pressure, temperature, salinity, and density and taxon or particle concentrations were calculated in 1-second intervals. Down and up casts were averaged by depth (1-m intervals) to yield a single profile. Thirty-three deployments of the AutoVPR were conducted, although data from only twenty-nine profiles are considered here (of the four deployments not considered, two were conducted far to the south, one was a single replicate, and one contained no data). Profiles were collected at most of the standard SBI stations regardless of the time of day. Daylight was present throughout all 24 hours for these dates. Over 61,000 in-focus images were collected from these profiles. Nine taxa or particle types were identified: copepods, diatom chains, decaying diatoms, larvaceans, benthic larvae (primarily polychaete and echinoderm), marine snow, medusae (hydromedusae and scyphomedusae not differentiated), nauplii (primarily copepod) and radiolarians. Decaying diatoms were diatom chains that were observed visually to have accumulated flocculent material along the rod but were still easily identified as diatom chains Data from rarely observed taxa (e.g., pteropods, chaetognaths, ostracods) are not presented. DATA FORMAT: There is a single data file for each cast using the AutoVPR. The data files are named according to the station number. Each data file contains 14 columns. There is a header row at the top that describes the variables. Columns contain as follows: Columns 1:2=cast location (Latitude, Longitude) in decimal degrees, Column 3 = pressure in dbars, Column 4 = Water temperature (°C), Column 5 = Salinity, Column 6 = Density (sigma-t units), Column 7 = copepods (#/L), Column 8 = diatom rods (#/L), Column 9=Decaying/Fuzzy Diatoms (#/L), Column 101 = Larvaceans (#/L), Column 11=Benthic Larvae (#/L), Column 12 = Marine Snow (#/L), Column 13= Medusae (#/L), Column 15=Nauplii (#/L), Column 14 = Radiolarians (#/L). An accompanying table contains details on the date, time, location, water depth, and tow depth for each profile. Times/dates are presented both in Alaska Time and GMT.