This data set contains the atmospheric DMS concentration, sea-to-air flux, and transfer velocity measurements from the NOAA R/V Ronald H. Brown, on the STRATUS cruise during VOCALS-REx from 20 October to 1 December 2008. The cruise was divided to two legs. The first leg spanned from approximately 20 October to 3 November, starting from Panama and going to WHOI buoy (85 deg. W, 20 deg. S) and arriving in Arica, Chile. The second leg left Arica on 10 November to the Southeast Pacific and returned to Arica on 1 December. The authors have previously used the APIMS with isotopically labeled standard (APIMS-ILS) technique to measure atmospheric DMS (Huebert et al., 2004; Blomquist et al., 2006, Yang et al., 2009; Huebert et al., 2010). All data are presented in tab-delimited text files that should be readable by most spreadsheet programs. Please see the readme for more information.